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KMID : 0380020100250060539
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Korean Journal of Biotechnology and Bioengineering
2010 Volume.25 No. 6 p.539 ~ p.546
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Optimization of Medium and Fermentation Conditions for Mass Production of Bacillus licheniformis SCD121067 by Statistical Experimental Design
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Jeong Yoo-Min
Lee Ju-Hee
Chung Hea-Jong
Chun Gie-Taek
Yun Soon-Il
Jeong Yong-Seob
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Abstract
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In this work, mass production of Bacillus licheniformis SCD121067 through medium optimization by statistical experimental method was studied. First, galactose, yeast extract and potassium phosphate dibasic were selected as carbon, nitrogen and phosphate sources for mass production of B. licheniformis SCD121067 by using one factor at a time method. Second, according to the result of Plackett-Burman experimental design, key factors was yeast extract and K2HPO. Finally, the response surface methodology was performed to obtain the optimum concentrations of two selected variables. The optimized medium composition consisted of 20 g/L galactose, 36 g/L yeast extract, 0.41 g/L K2HPO4, 0.25 g/L Na2CO3, 0.4g/L MgSO4 and 0.01g/L CaCl2. Dry cell weight (15.4 g/L) by optimum production medium were increased 10 times, as compared to that determined with basic production medium (1.5 g/L). Fermentation conditions were examined for the mass production of B. licheniformis. The effect of temperature, agitation speed, pH and aeration rate on the mass production of B. licheniformis were also studied in a batch fermenter which was carried out in a 2.5 L bioreactor with a working volume of 1.5 L containing optimized production medium. As a result, dry cell weight of batch culture was 30.7 g/L at 42℃, 300 rpm, pH 8.0 and 2 vvm.
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KEYWORD
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Bacillus licheniformis, fermentation, statistical experimental design
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